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Lipopolysaccharides from Escherichia coli 0111:B4

Code: L2630
Đóng gói: 10mg, 25mg, 100mg
Hãng sản xuất: Sigma
Thông tin sản phẩm

Lipopolysaccharides from Escherichia coli 0111:B4

purified by phenol extraction

  •  EC Number 297-473-0

  •  MDL number MFCD00164401

    Properties

    Related Categories Biochemicals and Reagents, Carbohydrates, Carbohydrates A to Z, Carbohydrates H-L, Complex Lipids,
    Glycolipids, Lipids, Lipopolysaccharides, Polysaccharide
    Quality Level   PREMIUM
    form   lyophilized powder
    purified by   phenol extraction
    storage temp.   2-8°C

    Description

    Frequently Asked Questions

    Frequently Asked Questions are available for this Product.

    Application

    Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

    Packaging

    10, 25, 100 mg in poly bottle

    Preparation Note

    The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

    Biochem/physiol Actions

    Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

    General description

    This product is phenol extracted from E. coli serotype O111:B4. The source strain is from a private collection. This LPS serotype has been used to stimulate B-cells and induce NOS in human hepatocytes.

    Safety Information

    Symbol 
    GHS07  GHS07
    Signal word 
    Warning
    Hazard statements 
    Personal Protective Equipment 
    Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
    RIDADR 
    NONH for all modes of transport
    WGK Germany 
    3
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