Application
Used for non-selective, efficient fluorescent labeling of glycans.
2-anthranilic acid (2-AA) and 2-amino benzamide (2-AB) may be
used for non-selective fluorescent labeling of N- and O-glycans.[4] AA may also be used for facile labeling of oligosaccharides (acidic and neutral) in a nonselective manner.[6]
Other Notes
Sales restrictions may apply
Legal Information
FLUKA is a registered trademark of Honeywell Specialty Chemicals Seelze GmbH
General description
Anthranilic acid (or o-amino-benzoic acid) is generally known as
the essential precursor of quaternary dihydrofuro- and
dihydropyranoquinoline alkaloids.[2] It is also an intermediate produced during the biosynthesis of tryptophan.[3]
2-anthranilic acid (2-AA) label is highly suitable for electrophoretic
separations by polyacrylamide gel electrophoresis and shows high
intrinsic intensity thereby facilitating sensitive analysis of protein
glycosylation.[4]
Highly fluorescent anthranilic acid (AA, 2-aminobenzoic acid) labeled
oligosaccharides can be easily analyzed by MALDI-TOF MS thereby enabling
rapid determination of oligosaccharide mass map of glycoproteins.[6]
Protocols & Applications
Methods of glycosylated protein analysis by Mass Spectrometry