Application
RPMI 1640 Medium was developed at Roswell Park Memorial
Institute in 1966 by Moore and his co-workers. A modification of McCoy′s
5A Medium, it was formulated to support lymphoblastoid cells in
suspension culture, but it has since been shown to support a wide
variety of cells that are anchorage dependent. Originally intended to be
used with a serum supplement, RPMI 1640 has been shown to support
several cell lines in the absence of serum. It has also been widely used
in fusion protocols and in the growth of hybrid cells.
Quantity
Formulated to contain 8.4 grams of powder per liter of medium.
Reconstitution
Supplement with 2.0 g/L sodium bicarbonate.
Protocols & Applications
ECACC Handbook-Fundamental Techniques in Cell Culture
Cryostor Cryopreservation Video Protocol
Mycoplasma PCR Detection Kit Video Protocol