Leukemia Inhibitory Factor from mouse

LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution, suitable for cell culture

Synonym: LIF

•  MDL number MFCD00283681

  Properties

  Related Categories	Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Core Bioreagents, Cytokines, Cytokines and Growth Factors, Cytokines, Growth Factors and Hormones, Cytokines, Growth Factors, Chemokines for Stem Cell Biology, Cytokines, Growth Factors, and Chemokines for Stem Cell Expansion, General Stem Cell Biology, Growth Factors, Growth Factors and Cytokines, Hematopoietic Cytokines, Hematopoietic Stem Cell Cytokine and Growth Factors, Hematopoietic Stem Cell Reagents, Leukemia Inhibitory Factor (LIF), Life Science Reagents for Cell Culture, Nerve Growth Factors and Cytokines, Neural Stem Cell Biology, Neural Stem Cell Reagents, Reagents and Supplements, Research Essentials, Stem Cell Biology, Stem Cell Expansion
  recombinant	expressed in E. coli
  assay	≥95% (SDS-PAGE)
  form	buffered aqueous solution
  quality	endotoxin tested
  suitability	suitable for cell culture
  storage temp.	2-8°C

  Description

  Frequently Asked Questions

  Frequently Asked Questions are available for this Product.

  Analysis Note

  The proliferative activity of mouse LIF is measured in culture using the M1 murine cell line.

  Physical form

  Solution in phosphate buffered saline containing 0.02% TWEEN^® 20

  Biochem/physiol Actions

  Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein originally described to inhibit the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Other activities were later identified to LIF, which is known by a variety of synonyms, including DIF, D-factor, DIA, DRF, CNDF, HILDA, HSF-III, and MLPLI. Human LIF exerts its actions through a receptor comprising a 190 kDa LIF-binding α-chain (130 kDa, mouse) and a 130 kDa signal-transducing β-chain (gp130), which is shared with CNTF, OSM, L-6 and IL-11. As such, it is a member of the gp130 family of the cytokine receptor superfamily. LIF receptors have been identified on several cells, including monocytes, liver, placenta and embryonic stem cells. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, depending on the source, but absence of glycosylation appears not to affect its biological activity. A single gene encodes LIF, which is secreted as a single chain glycoprotein containing 180 amino acids for human or mouse with a conserved disulfide bond. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.

  Legal Information

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